• Main FAQs
  • How accurate is Peptest?

    Peptest is a highly accurate diagnostic test with validated performance measures as indicated below:

    • Sensitivity: 95%
    • Specificity: 89%
    • Pepsin detection limit 16 ng/ml
  • Should I take the three sample test or the two sample test?

    We recommend that you take the 3 sample test as this gives you a much richer set of results to share with your doctor and additional information on when you are likely to reflux. Studies have shown that taking 2 samples at the right time and according to the instructions is an extremely accurate diagnostic test. The additional sample in the three test strategy is used to determine if a patient is prone to refluxing in a supine position (i.e. lying down) this is useful information for healthcare professionals and is used in determining how best to treat and manage the condition.

  • What should I do once I get my diagnosis?

    The exact treatment pathway you follow will depend on your symptoms, the results of your Peptest analysis and any other relevant information your healthcare team possess. When you have your reflux diagnosis from Peptest you should discuss the next steps with your healthcare team.

  • Should I stop taking antacids or H2 Receptor antagonist prior to taking samples?

    We recommend stopping taking all antacid like products and Gaviscon for 48 hours prior to taking sample for Peptest analysis.

  • Should I stop PPI therapy prior to taking samples?

    No they do not have to stop PPI therapy, Peptest will still detect pepsin in the presence of a PPI if pepsin is being refluxed.

  • Can patient have LPR symptoms but not have reflux?

    1. A patient can present with LPR like symptoms which are not necessarily caused by reflux and this is the reason why a negative pepsin result can be as important as a positive pepsin result to help us identity the reasons why patients are experiencing symptoms at that time. If you have a positive pepsin result this indicates that reflux has occurred and the reflux should be treated appropriately.

    2. However a negative pepsin result in a patient with LPR like symptoms is far more challenging. The symptoms may be due to i) a previous reflux event which has damaged the sensitive epithelium cell layer of the throat, vocal cords, airways ii) this damaged area has allowed bacteria to colonise which will cause inflammation and irritation and possibly needs to be treated with antibiotics on anti-inflammatory type medication iii) the symptoms might be the result of an allergy or food intolerance and this will need investigating iv) the damage to the delicate epithelial cell lining might have been caused by smoking or alcohol or by insulting the cell lining by drinking excessively hot or acidic drinks?

    3. One suggestion if symptoms persist and reflux has been ruled out by a negative Peptest result is for the patient to undergo a course of vocal/throat hygiene. A good ENT specialist can advise here or even better get in touch with a Speech Language Therapist attached to the ENT clinic.

  • Should I stop drinking alkaline water?

    Yes. Alkaline water (pH 8.8) has been demonstrated to denature pepsin in in-vitro studies. Although we know of no published data that exists to demonstrate whether or not alkaline water has any impact on therapeutic outcomes we recommend that alkaline water is avoided for 48 hours prior to taking the samples for Peptest.

General Questions

  • General Questions
  • What is Pepsin?

    Pepsin is a protease originating from pepsinogen secreted into gastric juice from chief cells and is only found in the stomach. It’s presence in the oesophagus or more proximally in the laryngopharyngeal region and airways suggests gastro oesophageal and extra oesophageal reflux

  • Is Reflux Disease Dangerous

    Reflux disease is a chronic disease which can in a small percentage of individuals lead to more serious conditions such as Barrett’s oesophagus, oesophageal adenocarcinoma and potentially laryngeal cancer.